H7915
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 | Immunoblotting Whole extract of MCF7 cells was separated on SDS-PAGE and probed with Rabbit Anti-HRD1/SYVN1(C-Terminal) (Cat. No. H7915). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 10 μg/mL antibody 2. 5 μg/mL antibody 3. 5 μg/mL antibody + HRD1/SYVN1 immunizing peptide (human, 589-604) |
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H7915
Sigma
Anti-HRD1/SYVN1 (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Synovial apoptosis inhibitor 1, Anti-Synoviolin 1, Anti-Synoviolin |
Description
| Immunogen | synthetic peptide corresponding to amino acids 589-604 of human HRD1/SYVN1, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse. |
| Physical form | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~70 kDa |
| species reactivity | human |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: 0.2-0.4 μg/mL using whole extract of HEK-293T cells expressing human HRD1/SYVN1. |
| immunoblotting: 5-10 μg/mL using whole extract of human MCF-7 cells. | |
| Gene Information | human ... SYVN1(84447) |
| rat ... Syvn1(361712) | |
| mouse ... Syvn1(74126) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 1 |
